General description

Sulfatases (EC 3.1.6) are a family of enzymes that catalyze the hydrolysis of sulfate ester bonds from a broad range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.

Principle

This Sulfatase Activity Assay Kit provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at 515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.

Suitability

Suitable for measuring activity of purified enzyme as well as sulfatase from biological samples.